Immunology: article synopsis
The article, “Extensive RNA Editing and Splicing Increase Self-Representation Diversity in Medullary Thymic Epithelial Cells,” by Danan-Gotthold (2016) describes the process of establishing a central tolerance in the thymus. The immature T cells are trained by the thymic stroma to be immunocompetent. The prepared cells become capable of identifying recognizing invaders. The process which involved instruction of the T cells to discriminate between self and non–self while being potentially harmless. The experiment aims to determine the levels of medullary thymic epithelial cells. The article uses various research methods to analyze and describe the findings from the experiments conducted. The present review will examine the experimental approach, justification, and significance of the research.
Experimental approach and justification
From the article, the researchers used various controlled measures to test their hypothesis. The hypothesis of the research aimed at proofing that extensive RNA editing and gene splicing would increase self-representation diversity in mTECs. The approaches included;
RNA sequencing
This process of RNA sequencing is used in the measurement of a transcriptome. The researchers placed three different gene sample populations through this procedure to achieve their findings; this included; mTECs1 one which included the population with immature genes, mTECs2; which was composed of mature gene population, and (AireKO); which comprised of a population with severe impaired promiscuity of gene expression. mTECs1 and mTECs2 differed in that mTECs1